Dissociation Studies, I.
نویسندگان
چکیده
منابع مشابه
STUDIES ON DISPERSED PANCREATIC EXOCRINE CELLS I. Dissociation Technique and Morphologic Characteristics of Separated Cells
A procedure for dissociation of the guinea pig pancreas into individual cells is described which employs enzymatic digestion with pure collagenase, chymotrypsin, and hyaluronidase, utilizes an interposed chelation of divalent cations by EDTA, and is terminated by gentle shearing. Yields of cells are 50-60%, based on DNA recovered. The population comprises ~95% exocrine cells, the remainder cons...
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The human erythrocyte contains a complex of peripheral membrane proteins which forms an extensive network or cytoskeleton on the cytoplasmic membrane surface. When I treat erythrocyte cytoskeletons with deoxyribonuclease I (DNase I), the cytoskeletons dissociate and erythrocyte actin is solubilized. The dissociation of the cytoskeletons by DNase I parallels the disruption of actin filaments in ...
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Electromechanical dissociation (EMD) is the most frequent cause of unsuccessful cardiac resuscitation in critically ill patients. In a clinical study of cardiac arrest, including 54 episodes in 50 fully monitored patients, 14 episodes of ventricular fibrillation were observed and seven were reversed. In the remaining 40 instances, 36 cases of EMD were initially observed. Four patients had asyst...
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ژورنال
عنوان ژورنال: The Journal of Physical Chemistry
سال: 1899
ISSN: 0092-7325,1541-5740
DOI: 10.1021/j150011a002